One of the most common methods of storing tissue is freezing the sample so that it can be kept for later use. This means that the samples are stored in a liquid nitrogen-cooled cryostat in a storage solution, however, this method is not without its challenges. This article explains these challenges and presents solutions for them as well.
Storage FFPE tissue samples can be used to carry out a wide range of applications and medical procedures. The main benefits of using storage paraffin-embedded tissue are that it is easy to use, inexpensive per procedure, and easily transported.
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The process of storage paraffin-embedded tissue works by being made up of three parts. The first part is the primary agent, which is usually nitrous oxide gas. Nitrous oxide gas is the largest component in the mixture and provides a large portion of the pressure for the process. The second main component is oxygen, and this occurs because there is no air inside a storage jar. The third main component in storage paraffin-embedded tissue is paraffin wax, which has most of its properties mimicked by various oils.
The storage of paraffin-embedded tissue (SPET) is a process that is used for the preservation and storage of tissues. The tissue sample is immersed in liquid paraffin, which hardens around the tissue and protects it from chemical deterioration. The tissue sample is then frozen using liquid nitrogen or dry ice. The sample is then embedded in a paraffin block, which hardens around the tissue and protects it from bacterial degradation. The tissue sample is then sectioned into thin sections, dehydrated, and embedded in plastic molds.